Natural Bio-active Compounds by Mohd Sayeed Akhtar & Mallappa Kumara Swamy

Author:Mohd Sayeed Akhtar & Mallappa Kumara Swamy
Language: eng
Format: epub
ISBN: 9789811374388
Publisher: Springer Singapore

(ii)Flow and mass transfer conditions in the bioreactor

(iii)Kinetics of cell growth and product formation

(iv)Genetic stability of productive cell lines

(v)Control of micro- and macroenvironment in the bioreactor

(vi)Implications of bioreactor design on downstream processing

(vii)Potential for process scale-up

Bioreactor operation can be batch, fed-batch, or continuous. Batch bioreactors are used to regulate optimum production conditions upon scale-up from small-scale fermentations in a flask. If the cell culture is under the impact of limiting nutrient, fed-batch operation is favored. The usual operation mode after optimization studies is the continuous mode or the chemo state which allows continuous supply of the nutrient medium and removal of the products allowing a steady state operation. If secondary metabolite biosynthesis is growth-related, a single-step bioreactor is sufficient. Elseways, stagewise fermentation is proposed where the first bioreactor is used for culture growth and the second one is used for secondary metabolite biosynthesis (Payne et al. 1993). Intracellular products usually require batch or fed-batch operations, while extracellular products allow continuous production schemes.

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